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Fibronectin antibody

Anti-Fibronectin antibody used in IHC (Paraffin sections) (IHC-P). GTX72724

Cat. No. GTX72724

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, IHC

Reactivity

 Human, Pig
Package
500 μl ($399)
See all Fibronectin products

APPLICATION

Application Note

Immunohistochemistry (IHC) 1:100-1:200 for 30 minute at room temperature using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears require antigen retriever, boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temperature for 20 minutes.

Calculated MW

263 kDa. ( Note )

Positive Control

Human kidney

PROPERTIES

Form

Liquid

Buffer

10mM PO₄, 150mM NaCl, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human Fibronectin from human plasma.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_374816

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibronectin 1 , CIG , ED-B , FINC , FN , FNZ , GFND , GFND2 , LETS , MSF , SMDCF

Background

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Fibronectin antibody used in IHC (Paraffin sections) (IHC-P). GTX72724

GTX72724 IHC-P Image

The data was published in the journal Biomed Res Int in 2015. PMID: 25918720

REFERENCE

Application Reference

Jong AY et al. J Extracell Vesicles 2017; 6 (1) : 1294368 Large-scale isolation and cytotoxicity of extracellular vesicles derived from activated human natural killer cells.
Bao J et al. Int J Mol Med 2016; 38 (2) : 457-65 Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold.
Application : IHC-P
Reactivity : Pig
Bao J et al. Sci Rep 2015; 5 : 10756 Hemocompatibility improvement of perfusion-decellularized clinical-scale liver scaffold through * immobilization.
Application : IHC
Reactivity : Pig
Wu Q et al. Biomed Res Int 2015; 2015 : 785474 Optimizing perfusion-decellularization methods of porcine livers for clinical-scale whole-organ bioengineering.
Application : IHC
Reactivity : Pig
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399