APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Flightless I.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
FLII actin remodeling protein , FLI , FLIL , Fli1
Cellular Localization
Nucleus,Cytoplasm, cytoskeleton,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Cell junction, focal adhesion
Background
This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Flightless I antibody [HL2966] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640353](/templates/default/js/lightbox/images/loading.gif "Anti-Flightless I antibody [HL2966] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640353")
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GTX640353 ICC/IF Image
Flightless I antibody [HL2966] detects Flightless I protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Flightless I stained by Flightless I antibody [HL2966] (GTX640353) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX640353 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Flightless I antibody [HL2966] (GTX640353) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Flightless I antibody [HL2966] (GTX640353). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Flightless I antibody [HL2966] (GTX640353). Be the first to share your experience with this product.
![Anti-Flightless I antibody [HL2966] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640353](/upload/website/prouct_img/small/GTX640353/GTX640353_T-45411_20240607_ICC_IF_24062501_689.webp "Anti-Flightless I antibody [HL2966] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640353")
![Anti-Flightless I antibody [HL2966] used in Western Blot (WB). GTX640353](/upload/website/prouct_img/small/GTX640353/GTX640353_T-45411_20240621_WB_24062501_854.webp "Anti-Flightless I antibody [HL2966] used in Western Blot (WB). GTX640353")
![Anti-Flightless I antibody [HL2966] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640353](/upload/website/prouct_img/normal/GTX640353/GTX640353_T-45411_20240607_ICC_IF_24062501_689.webp "Anti-Flightless I antibody [HL2966] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640353")
![Anti-Flightless I antibody [HL2966] used in Western Blot (WB). GTX640353](/upload/website/prouct_img/normal/GTX640353/GTX640353_T-45411_20240621_WB_24062501_854.webp "Anti-Flightless I antibody [HL2966] used in Western Blot (WB). GTX640353")