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GAD67 antibody [3H2]

Overexpression
Anti-GAD67 antibody [3H2] used in Immunoprecipitation (IP). GTX84470
Anti-GAD67 antibody [3H2] used in IHC (Paraffin sections) (IHC-P). GTX84470
Anti-GAD67 antibody [3H2] used in Western Blot (WB). GTX84470
Anti-GAD67 antibody [3H2] used in IHC (Paraffin sections) (IHC-P). GTX84470
Anti-GAD67 antibody [3H2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84470
Anti-GAD67 antibody [3H2] used in Western Blot (WB). GTX84470
Anti-GAD67 antibody [3H2] used in IHC (Paraffin sections) (IHC-P). GTX84470
Anti-GAD67 antibody [3H2] used in IHC (Paraffin sections) (IHC-P). GTX84470
Anti-GAD67 antibody [3H2] used in IHC (Paraffin sections) (IHC-P). GTX84470

Cat. No. GTX84470

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3H2

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Dog
Package
100 μl ($519)
See all GAD67 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
IHC-P 1:50
IP 2ug/500ul
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GAD1 (NP_000808) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate decarboxylase 1 , CPSQ1 , GAD , SCP

Background

Catalyzes the production of GABA.

Database

Research Area

DATA IMAGES

Anti-GAD67 antibody [3H2] used in Immunoprecipitation (IP). GTX84470

GTX84470 IP Image

IP analysis of DDDDK tagged GAD67 overexpressed HEK293T lysate using GTX84470 GAD67 antibody [3H2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-GAD67 antibody [3H2] used in IHC (Paraffin sections) (IHC-P). GTX84470

GTX84470 IHC-P Image

IHC-P analysis of human colon tissue using GTX84470 GAD67 antibody [3H2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-GAD67 antibody [3H2] used in Western Blot (WB). GTX84470

GTX84470 WB Image

WB analysis of various cell lines using GTX84470 GAD67 antibody [3H2].
Loading : 35 ug per lane

Anti-GAD67 antibody [3H2] used in IHC (Paraffin sections) (IHC-P). GTX84470

GTX84470 IHC-P Image

IHC-P analysis of human breast tissue using GTX84470 GAD67 antibody [3H2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-GAD67 antibody [3H2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84470

GTX84470 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with GAD67 plasmid using GTX84470 GAD67 antibody [3H2].

Anti-GAD67 antibody [3H2] used in Western Blot (WB). GTX84470

GTX84470 WB Image

Overexpression

WB analysis of HEK293T cells transfected with GAD67 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84470 GAD67 antibody [3H2].
Loading : 5 ug per lane

Anti-GAD67 antibody [3H2] used in IHC (Paraffin sections) (IHC-P). GTX84470

GTX84470 IHC-P Image

IHC-P analysis of human bladder tissue using GTX84470 GAD67 antibody [3H2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-GAD67 antibody [3H2] used in IHC (Paraffin sections) (IHC-P). GTX84470

GTX84470 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84470 GAD67 antibody [3H2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-GAD67 antibody [3H2] used in IHC (Paraffin sections) (IHC-P). GTX84470

GTX84470 IHC-P Image

IHC-P analysis of human lung tissue using GTX84470 GAD67 antibody [3H2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519