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GAMT antibody

Anti-GAMT antibody used in Western Blot (WB). GTX33215
Anti-GAMT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33215
Anti-GAMT antibody used in IHC (Paraffin sections) (IHC-P). GTX33215

Cat. No. GTX33215

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all GAMT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-236 of human GAMT (NP_000147.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

guanidinoacetate N-methyltransferase , CCDS2 , HEL-S-20 , PIG2 , TP53I2

Background

The protein encoded by this gene is a methyltransferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl donor. Defects in this gene have been implicated in neurologic syndromes and muscular hypotonia, probably due to creatine deficiency and accumulation of guanidinoacetate in the brain of affected individuals. Two transcript variants encoding different isoforms have been described for this gene. Pseudogenes of this gene are found on chromosomes 2 and 13. [provided by RefSeq, Feb 2012]

Database

DATA IMAGES

Anti-GAMT antibody used in Western Blot (WB). GTX33215

GTX33215 WB Image

WB analysis of various sample lysates using GTX33215 GAMT antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GAMT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33215

GTX33215 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33215 GAMT antibody.
Blue : DAPI

Anti-GAMT antibody used in IHC (Paraffin sections) (IHC-P). GTX33215

GTX33215 IHC-P Image

IHC-P analysis of human liver tissue using GTX33215 GAMT antibody.
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399