Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
IHC-P
Endogenous peroxidase activity was blocked by incubation with 1.1% H2O2 for 10 minutes. Nonspecific binding of the second antibody was avoided by preincubation with normal rabbit serum for 15 minutes.
IHC-Fr
Endogenous peroxidase activity was blocked by incubation with 1.1% H2O2 for 10 minutes. Nonspecific binding of the second antibody was avoided by preincubation with normal rabbit serum for 15 minutes.
Neutralizing/Inhibition
The monoclonal antibody B2C10 inhibits the binding of 125I-labeled galectin-3 to IgE coated on microtiter plates, the galecin-3’s hemagglutination activity and galectin-3-induced superoxide production by human neutrophils.
|
Not tested in other applications.
Calculated MW
Product Note
The epitope of the monoclonal antibody B2C10 is found within the first 45 amino acids of galectin-3. The antibody B2C10 does not react with Galecin-3C.
Form
Liquid
Buffer
Filter-sterilized PBS, 0.1% BSA
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human Galectin-3
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
CBP35,GAL3,GALBP,GALIG,L31,LGALS2,LGALS3,MAC2,galectin 3,Galectin 3,Galectin3
Cellular Localization
Cytoplasm,Nucleus,Secreted
Background
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
Database
Research Area
