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Galectin 4 antibody

Overexpression
Anti-Galectin 4 antibody used in Western Blot (WB). GTX127351
Anti-Galectin 4 antibody used in Western Blot (WB). GTX127351
Anti-Galectin 4 antibody used in Western Blot (WB). GTX127351
Anti-Galectin 4 antibody used in Western Blot (WB). GTX127351

Cat. No. GTX127351

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Galectin 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HL-60 , LGALS4 transfected 293T , HCT116 , mouse colon

Predict Reactivity

Rabbit, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galectin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885645

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galectin 4 , GAL4 , L36LBP

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Galectin 4 antibody used in Western Blot (WB). GTX127351

GTX127351 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galectin4 antibody (GTX127351) diluted at 1:500.

Anti-Galectin 4 antibody used in Western Blot (WB). GTX127351

GTX127351 WB Image

Overexpression

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: LGALS4 transfected 293T lysate
10% SDS PAGE
GTX127351 diluted at 1:5000

Anti-Galectin 4 antibody used in Western Blot (WB). GTX127351

GTX127351 WB Image

Sample (30 ug of whole cell lysate)
A: HL-60
10% SDS PAGE
GTX127351 diluted at 1:1000

Anti-Galectin 4 antibody used in Western Blot (WB). GTX127351

GTX127351 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galectin4 antibody (GTX127351) diluted at 1:500.

REFERENCE

Application Reference

Tzeng SF et al. FASEB J 2018; : fj201800687 O-Glycosylation-mediated signaling circuit drives metastatic castration-resistant prostate cancer.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169