Gelsolin antibody [C2C3], C-term

Name: Gelsolin antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX101185
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Gelsolin antibody [C2C3], C-term used in Western Blot (WB). GTX101185

Anti-Gelsolin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101185

Anti-Gelsolin antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101185

Cat. No. GTX101185

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Gelsolin products

View product citations for antibody GTX101185 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

H1299 , HeLa , human plasma , rat plasma , mouse plasma

Predict Reactivity

Sheep, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Gelsolin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950463

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gelsolin , ADF , AGEL

Cellular Localization

Isoform 2: Cytoplasm , cytoskeleton , Isoform 1: Secreted

Background

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 2934 GSN
UniProt: P06396 GSN

Research Area

DATA IMAGES

GTX101185 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.

GTX101185 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.

GTX101185 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Gelsolin(GTX101185) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101185 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Gelsolin(GTX101185) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101185 WB Image

Gelsolin antibody detects Gelsolin protein by western blot analysis. Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody (GTX101185) diluted at 1:1000.

GTX101185 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
7.5% SDS PAGE
GTX101185 diluted at 1:1000

GTX101185 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.

REFERENCE

Application Reference

Fan SM et al. Biomaterials 2018; 167 : 121-131 Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin.

Cheng CW et al. Cancer Med 2017; (Epub) Sex hormone-binding globulin (SHBG) is a potential early diagnostic biomarker for gastric cancer.

Application : WB

Reactivity : Human

Yang HH et al. Proteomics Clin Appl 2016; (Epub) the putative involvement of actin binding proteins and cytoskeleton proteins in pathological mechanisms of * cystitis - revealed by a prospective pilot study using proteomic approaches.

Application : WB

Reactivity : Human

Law CH et al. Toxicology 2013; 303 : 54-71 Hyaluronic acid-dependent protection in H9C2 cardiomyocytes: a cell model of heart ischemia-reperfusion injury and treatment.

Application : WB

Reactivity : Rat

Lu CH et al. Arch Biochem Biophys 2013; 529 (2) : 146-56 Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients.

Application : WB

Reactivity : Human

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