APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
MCF-7 , MDA-MB-231
Product Note
Specific for GAC but not KGA isoform
Predict Reactivity
Mouse, Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutaminase C (GAC). The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888301
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutaminase , AAD20 , CASGID , EIEE71 , GAC , GAM , GLS1 , KGA
Cellular Localization
Mitochondrion
Background
Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]
Database
Research Area
DATA IMAGES
|
GTX632265 ICC/IF Image
Glutaminase C antibody [GT1075] detects Glutaminase C protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutaminase C protein stained by Glutaminase C antibody [GT1075] (GTX632265) diluted at 1:400. Blue: Hoechst 33342 staining.
|
|
GTX632265 WB Image
Glutaminase C (GAC) antibody detects Glutaminase C (GAC) protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody (GTX632265) diluted at a dilution of 1:1000.
|
REFERENCE
There are currently no references for Glutaminase C (GAC) antibody [GT1075] (GTX632265). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Glutaminase C (GAC) antibody [GT1075] (GTX632265). Be the first to share your experience with this product.