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HINT1 antibody

Anti-HINT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17017
Anti-HINT1 antibody used in Western Blot (WB). GTX17017

Cat. No. GTX17017

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all HINT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

HINT1 antibody was raised against a 17 amino acid synthetic peptide near the center of the human HINT1.The immunogen is located within the last 50 amino acids of HINT1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histidine triad nucleotide binding protein 1 , HINT , NMAN , PKCI-1 , PRKCNH1

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. This gene has been found to be a tumor suppressing gene. Several transcript variants, but only one of them protein-coding, have been found for this gene. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-HINT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17017

GTX17017 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX17017 HINT1 antibody.
Working concentration : 2.5 μg/ml

Anti-HINT1 antibody used in Western Blot (WB). GTX17017

GTX17017 WB Image

WB analysis of Jurkat cell lysate using GTX17017 HINT1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399