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HP1 alpha antibody

KO/KD
Anti-HP1 alpha antibody used in Western Blot (WB). GTX53906
Anti-HP1 alpha antibody used in Immunoprecipitation (IP). GTX53906
Anti-HP1 alpha antibody used in Western Blot (WB). GTX53906
Anti-HP1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53906

Cat. No. GTX53906

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399)
See all HP1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human CBX5 (NP_001120794.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromobox 5 , HEL25 , HP1 , HP1A

Cellular Localization

Nucleus

Background

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HP1 alpha antibody used in Western Blot (WB). GTX53906

GTX53906 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX53906 HP1 alpha antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HP1 alpha antibody used in Immunoprecipitation (IP). GTX53906

GTX53906 IP Image

IP analysis of HeLa cell lysate using GTX53906 HP1 alpha antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-HP1 alpha antibody used in Western Blot (WB). GTX53906

GTX53906 WB Image

WB analysis of various sample lysates using GTX53906 HP1 alpha antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HP1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53906

GTX53906 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX53906 HP1 alpha antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399