Application Note
Recommended Starting Dilutions:
For WB: 1:500-1:3000
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.
Calculated MW
Positive Control
HeLa , Blocking peptide (GTX70017-PEP) , HeLa (100 ng/ml Nocodazole treatment for 24 hr)
Predict Reactivity
Human (100%)| Mouse (93%)| Bovine (86%)| Rhesus Monkey (100%)
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.26 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser55 of human Hec1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11162004
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80
Cellular Localization
Nucleus , Kinetochore
Background
HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]
Database
Research Area