APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
HCT116 (30μM cisplatin treatment for 24hr) , NIH-3T3 (30μM cisplatin treatment for 24hr) , PC-12 (30uM cisplatin treatment for 24h) , U2OS treated UV (50 J/m2)
Predict Reactivity
Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.92 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of human Histone H2A.X. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888051
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
,H2A histone family member X,gamma H2A.X,gamma H2AX,H2A.X,H2A/X,H2AX,H2AFX,
Cellular Localization
Nucleus
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX628789 IP Image
Histone H2A.X (phospho S139) antibody immunoprecipitates histone H2A.X (phospho S139) protein in IP experiments. IP Sample: 500 μg HCT116 with CPT 30 μM treatment 24 hr whole cell lysate/extract A. 30 μg HCT116 whole with CPT 30 uM treatment cell lysate/extract B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of histone H2A.X (phospho S139) protein by 2 μg histone H2A.X (phospho S139) antibody (GTX628789) 15% SDS-PAGE The immunoprecipitated histone H2A.X (phospho S139) protein was detected by Human histone H2A.X (phospho S139) antibody (GTX628789) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
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GTX628789 WB Image
Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis. A. 30 μg NIH-3T3 whole cell lysate/extract (untreated) B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr) 15% SDS-PAGE Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628789 IHC-P Image
Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628789 ICC/IF Image
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis. Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Red: H2A.X protein stained by Histone H2A.X (phospho Ser139) antibody (GTX628789) diluted at 1:500. Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]
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GTX628789 WB Image
Histone H2A.X (phospho S139) antibody [GT2311] detects Histone H2A.X (phospho S139) [GT2311] protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628789 ICC/IF Image
Histone H2A.X antibody detects H2AFX protein at nuclear by immunofluorescent analysis. Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.Xantibody (GTX628789) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX628789 WB Image
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 50 J/m2 UV treatment) U2OS whole cell extracts (16 μg) were separated by 12%-15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628789 WB Image
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX628789 WB Image
The data was published in the 2019 in Int J Mol Sci. PMID: 31717306
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GTX628789 ICC/IF Image
The data was published in the 2019 in Int J Mol Sci. PMID: 31717306
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GTX628789 WB Image
The data was published in the journal Sci Rep in 2020.PMID: 31980707
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GTX628789 WB Image
The data was published in the journal PLoS Negl Trop Dis in 2017. PMID: 28783752
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GTX628789 WB Image
The data was published in the journal Front Physiol in 2019. PMID: 31681015
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REFERENCE
REVIEW
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