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Histone H2AK5ac (acetyl Lys5) antibody

Orthogonal
Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Western Blot (WB). GTX128961
Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Immunoprecipitation (IP). GTX128961
Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128961
Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Dot blot (Dot). GTX128961

Cat. No. GTX128961

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, Dot

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Histone H2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IP 1:100-1:10000
Dot Assay dependent
Not tested in other applications.

Positive Control

HeLa (0.012% DMSO treatment for 18 hr) , HeLa (0.4 μM Trichostatin A treatment for 18 hr)

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys5 of human Histone H2A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885849

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Western Blot (WB). GTX128961

GTX128961 WB Image

Orthogonal

Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Trichostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) dilution: 1:10000

Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Immunoprecipitation (IP). GTX128961

GTX128961 IP Image

Histone H2AK5ac (acetyl Lys5) antibody immunoprecipitates Histone H2AK5ac (acetyl Lys5) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H2AK5ac (acetyl Lys5) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H2AK5ac (acetyl Lys5) protein by 2.5 μg of Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) 12% SDS-PAGE The immunoprecipitated Histone H2AK5ac (acetyl Lys5) protein was detected by Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) diluted at 1 : 10000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128961

GTX128961 ICC/IF Image

Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 0.4 μM TSA for 18 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2AK5ac (acetyl Lys5) protein stained by Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Dot blot (Dot). GTX128961

GTX128961 Dot Image

Dotblot analysis of anti-Histone H2AK5ac (acetyl Lys5) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) 1:5000 dilution.
A: Peptide samples of H2A.2K5 un
B: Peptide samples of H2A.2K5ac
C: Peptide samples of H2BK20 un
D: Peptide samples of H2BK20ac
E: Peptide samples of H2BK5 un
F: Peptide samples of H2BK5ac

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169