Histone H3K27ac (Acetyl Lys27) antibody [RM172]

Cat. No. GTX60887

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM172
Isotype	IgG
Application	WB, ICC/IF, ELISA, ChIP assay
Reactivity	Human
Package	100 μg ($449)

See all Histone H3 products

Datasheet

View product citations for antibody GTX60887 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.5 μg/mL - 2 μg/mL
ICC/IF	0.5 μg/mL - 2 μg/mL
ELISA	0.2 μg/mL - 1 μg/mL
ChIP assay	Assay dependent

Not tested in other applications.

Product Note

This antibody reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with other acetylated Lysines in histone H3.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys27).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60887 WB Image

WB analysis of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) using GTX60887 Histone H3K27ac (Acetyl Lys27) antibody [RM172].
Dilution : 1μg/ml

GTX60887 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60887 Histone H3K27ac (Acetyl Lys27) antibody [RM172].
Red : Primary antibody
Green : Actin

GTX60887 Image

The GTX60887 reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 449

Qty

Histone H3K27ac (Acetyl Lys27) antibody [RM172]

Cat. No. GTX60887

Host

Clonality

Clone Name

Isotype

Application

Reactivity