Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175]

Cat. No. GTX60892

Reference 7

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM175
Isotype	IgG
Application	WB, ICC/IF, ELISA, ChIP assay, ChIP-seq, IHC
Reactivity	Human, Mouse
Package	100 μg ($449)

See all Histone H3 products

Datasheet
Reference

View product citations for antibody GTX60892 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1 μg/mL - 2 μg/mL
ICC/IF	Assay dependent
ELISA	0.5μg/mL - 1 μg/mL
ChIP assay	Assay dependent
ChIP-seq	Assay dependent
IHC	0.5 μg/mL - 2 μg/mL

Not tested in other applications.

Product Note

This antibody reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888006

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60892 Image

The GTX60892 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2),or other methylations in Histone H3.

GTX60892 WB Image

WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60892 Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175].
Dilution : 1μg/ml

GTX60892 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60892 Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175].

GTX60892 ICC/IF Image

The data was published in the journal Nat Commun in 2019.PMID: 31270318

REFERENCE

Application Reference

Andrea J Kriz et al. Mol Cell 2021; 81 (9) : 1970-1987.e9 Balancing cohesin eviction and retention prevents aberrant chromosomal interactions, Polycomb-mediated repression, and X-inactivation.

Application : ChIP-seq

Tchasovnikarova IA et al. Mol Cell 2021; (Epub) TRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways.

Reactivity : Human

Colognori D et al. Dev Cell 2020; 54 (1) : 21-32.e5 Xist Repeats A and B Account for Two Distinct Phases of X Inactivation Establishment.

Application : ChIP assay

Reactivity : Mouse

Wang CY et al. Nat Commun 2019; 10 (1) : 2950 PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments.

Application : ICC/IF

Reactivity : Mouse

Colognori D et al. Mol Cell 2019; 74 (1) : 101-117.e10 Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X.

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REVIEW

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Application Reference

Andrea J Kriz et al. Mol Cell 2021; 81 (9):1970-1987.e9 Balancing cohesin eviction and retention prevents aberrant chromosomal interactions, Polycomb-mediated repression, and X-inactivation.

Application : ChIP-seq

Tchasovnikarova IA et al. Mol Cell 2021; (Epub) TRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways.

Reactivity : Human

Colognori D et al. Dev Cell 2020; 54 (1):21-32.e5 Xist Repeats A and B Account for Two Distinct Phases of X Inactivation Establishment.

Application : ChIP assay

Reactivity : Mouse

Wang CY et al. Nat Commun 2019; 10 (1):2950 PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments.

Application : ICC/IF

Reactivity : Mouse

Colognori D et al. Mol Cell 2019; 74 (1):101-117.e10 Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X.

Wang CY et al. Cell 2018; 174 (2):406-421.e25 SMCHD1 Merges Chromosome Compartments and Assists Formation of Super-Structures on the Inactive X.

Sunwoo H et al. Proc Natl Acad Sci U S A 2017; (Epub) Repeat E anchors Xist RNA to the inactive X chromosomal compartment through CDKN1A-interacting protein (CIZ1).

Application : ICC/IF

Reactivity : Human

Package

List Price ($)

100 μg

$ 449

Qty

Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175]

Cat. No. GTX60892

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Research Area

DATA IMAGES

GTX60892 Image

GTX60892 WB Image

GTX60892 ICC/IF Image

GTX60892 ICC/IF Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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