Histone H3K9ac (Acetyl Lys9) antibody [RM161]

Cat. No. GTX60885

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM161
Isotype	IgG
Application	WB, ICC/IF, ELISA, ChIP assay, IHC, Sandwich ELISA
Reactivity	Human
Package	100 μg ($449)

See all Histone H3 products

Datasheet

View product citations for antibody GTX60885 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.25 μg/mL - 1 μg/mL
ICC/IF	0.5 μg/mL - 2 μg/mL
ELISA	0.2 μg/mL - 1 μg/mL
ChIP assay	2 μg/mL - 10 μg/mL
IHC	0.1 μg/mL - 1 μg/mL
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX60888, Detection : GTX60885. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.

Not tested in other applications.

Product Note

This antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys9).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60885 WB Image

WB analysis of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161].
Dilution : 0.25μg/ml

GTX60885 ChIP assay Image

ChIP analysis of HeLa cells using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg

GTX60885 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161].
Red : Primary antibody
Green : Actin

GTX60885 ELISA Image

Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody and biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.

GTX60885 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161].

GTX60885 Image

The GTX60885 reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetyl-ated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

GTX60885 ELISA Image

Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 449

Qty

Histone H3K9ac (Acetyl Lys9) antibody [RM161]

Cat. No. GTX60885

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Research Area

DATA IMAGES

GTX60885 WB Image

GTX60885 ChIP assay Image

GTX60885 ICC/IF Image

GTX60885 ELISA Image

GTX60885 ICC/IF Image

GTX60885 Image

GTX60885 ELISA Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.