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Histone H3S10ph (phospho Ser10) antibody [GT1195]

Recombinant antibody Orthogonal
Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in Western Blot (WB). GTX00957
Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in IHC (Paraffin sections) (IHC-P). GTX00957
Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in Western Blot (WB). GTX00957
Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00957
Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in Western Blot (WB). GTX00957
Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in Western Blot (WB). GTX00957
Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00957

Cat. No. GTX00957

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1195

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Phospho-Histone H3 (S10).

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in Western Blot (WB). GTX00957

GTX00957 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody [GT1195] (GTX00957) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in IHC (Paraffin sections) (IHC-P). GTX00957

GTX00957 IHC-P Image

IHC-P analysis of human oophoroma tissue section using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Dilution : 1:100

Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in Western Blot (WB). GTX00957

GTX00957 WB Image

Orthogonal

WB analysis of NIH-3T3 cell extracts with/without nocodazole (50 ng/ml, 37ºC, 20 hrs) treatment or CIP (20μl/400μl, 37ºC, 1 hr) treatment using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Dilution : 1:1000
Loading : 25μg

Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00957

GTX00957 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Orange : Primary antibody
Blue : DAPI

Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in Western Blot (WB). GTX00957

GTX00957 WB Image

Orthogonal

WB analysis of C6 cell extracts with/without nocodazole (50 ng/ml, 37ºC, 20 hrs) treatment or CIP (20μl/400μl, 37ºC, 1 hr) treatment using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Dilution : 1:1000
Loading : 25μg

Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in Western Blot (WB). GTX00957

GTX00957 WB Image

Orthogonal

WB analysis of HeLa cell extracts with/without nocodazole (50 ng/ml, 37ºC, 20 hrs) treatment or CIP (20μl/400μl, 37ºC, 1 hr) treatment using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Dilution : 1:1000
Loading : 25μg

Anti-Histone H3S10ph (phospho Ser10) antibody [GT1195] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00957

GTX00957 ICC/IF Image

ICC/IF analysis of PFA-fixed C6 cells using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Orange : Primary antibody
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399