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Histone H3S10ph (phospho Ser10) antibody [GT921]

Orthogonal
Anti-Histone H3S10ph (phospho Ser10) antibody [GT921] used in IHC (Paraffin sections) (IHC-P). GTX630185
Anti-Histone H3S10ph (phospho Ser10) antibody [GT921] used in Western Blot (WB). GTX630185
Anti-Histone H3S10ph (phospho Ser10) antibody [GT921] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630185
Anti-Histone H3S10ph (phospho Ser10) antibody [GT921] used in IHC (Paraffin sections) (IHC-P). GTX630185
Anti-Histone H3S10ph (phospho Ser10) antibody [GT921] used in IHC (Paraffin sections) (IHC-P). GTX630185

Cat. No. GTX630185

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT921

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

HeLa (100 ng/ml Nocodazole treatment for 24 hr)

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser10 of human Histone H3. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888190

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3S10ph (phospho Ser10) antibody [GT921] used in IHC (Paraffin sections) (IHC-P). GTX630185

GTX630185 IHC-P Image

Histone H3S10ph (phospho Ser10) antibody [GT921] detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [GT921] (GTX630185) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3S10ph (phospho Ser10) antibody [GT921] used in Western Blot (WB). GTX630185

GTX630185 WB Image

Orthogonal

Histone H3 (phospho Ser10) antibody [GT921] detects Histone H3 (phospho Ser10) protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated) |
B. 30 μg HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr)
15 % SDS-PAGE
Histone H3 (phospho Ser10) antibody [GT921] (GTX630185) dilution: 1:1000

Anti-Histone H3S10ph (phospho Ser10) antibody [GT921] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630185

GTX630185 ICC/IF Image

Histone H3S10ph (phospho Ser10) antibody [GT921] detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunofluorescent analysis.
Samples: HCT116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p21 Cip1 protein stained by p21 Cip1 antibody (GTX100444) diluted at 1:1000.
Red: Histone H3S10ph (phospho Ser10) protein stained by Histone H3S10ph (phospho Ser10) antibody [GT921] (GTX630185) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Histone H3S10ph (phospho Ser10) antibody [GT921] used in IHC (Paraffin sections) (IHC-P). GTX630185

GTX630185 IHC-P Image

Histone H3S10ph (phospho Ser10) antibody [GT921] detects Histone H3S10ph (phospho Ser10) protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Histone H3S10ph (phospho Ser10) antibody [GT921] (GTX630185) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3S10ph (phospho Ser10) antibody [GT921] used in IHC (Paraffin sections) (IHC-P). GTX630185

GTX630185 IHC-P Image

Histone H3S10ph (phospho Ser10) antibody [GT921] detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [GT921] (GTX630185) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations