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Histone H4 antibody - ChIP grade

Anti-Histone H4 antibody - ChIP grade used in Western Blot (WB). GTX60345
Anti-Histone H4 antibody - ChIP grade used in ELISA (ELISA). GTX60345
Anti-Histone H4 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60345
Anti-Histone H4 antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60345

Cat. No. GTX60345

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, ChIP assay

Reactivity

 Human
Package
50 μg ($429)
See all Histone H4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Histone H4 using a KLH-conjugated synthetic peptide containing an unmodified sequence from the central part of the protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H4 antibody - ChIP grade used in Western Blot (WB). GTX60345

GTX60345 WB Image

WB analysis of whole cell extracts (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60345 Histone H4 antibody - ChIP grade.
Dilution : 1:1,000

Anti-Histone H4 antibody - ChIP grade used in ELISA (ELISA). GTX60345

GTX60345 ELISA Image

ELISA analysis of peptides used for immunization using GTX60345 Histone H4 antibody - ChIP grade.

Anti-Histone H4 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60345

GTX60345 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60345 Histone H4 antibody - ChIP grade. A titration consisting of 0.5, 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and c-fos genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-Histone H4 antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60345

GTX60345 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60345 Histone H4 antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000

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Package List Price ($)
$ 429