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Human GAS6 protein, His tag (active)

Human GAS6 protein, His tag (active). GTX00106-pro
Human GAS6 protein, His tag (active). GTX00106-pro
Human GAS6 protein, His tag (active). GTX00106-pro
Human GAS6 protein, His tag (active). GTX00106-pro

Cat. No. GTX00106-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($319)
See all GAS6 protein products

APPLICATION

Application Note

Growth arrest-specific 6, also known as GAS6, is a gamma-carboxyglutamic acid (Gla) domain-containing protein thought to be involved in the stimulation of cell proliferation. It has been reported that both PC-3 and DU 145 human prostate cancer cell lines are stimulated to proliferate by Gas6, however, this proliferative response strictly correlates with the expression of the Axl receptor, being higher in DU 145 cells. To test the proliferative effect of Gas6, DU 145 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of GAS6. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Cell proliferation of DU145 cells after incubation with GAS6 for 72h observed by inverted microscope.

Observed MW

24 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu136~Phe311 (NP_000811.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

growth arrest specific 6 , AXLLG , AXSF

Background

This gene encodes a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. [provided by RefSeq, Aug 2014]

Database

Research Area

DATA IMAGES

Human GAS6 protein, His tag (active). GTX00106-pro

GTX00106-pro Functional Assay Image

The dose effect curve of GTX00106-pro Human GAS6 protein (active) on DU145 cells. After incubation of Human GAS6 protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Human GAS6 protein, His tag (active). GTX00106-pro

GTX00106-pro Functional Assay Image

Cell proliferation effect of GTX00106-pro Human GAS6 protein (active).
(A) Unstimulated DU145 cells cultured in serum-free DMEM for 72hrs.
(B) DU145 cells cultured in serum-free DMEM, stimulated with 100 ng/ml Gas6 for 72hrs.

Human GAS6 protein, His tag (active). GTX00106-pro

GTX00106-pro Image

WB analysis of GTX00106-pro Human GAS6 protein (active).

Human GAS6 protein, His tag (active). GTX00106-pro

GTX00106-pro Image

SDS-PAGE analysis of GTX00106-pro Human GAS6 protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 319