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IDE antibody [GT143]

KO/KD
Anti-IDE antibody [GT143] used in Western Blot (WB). GTX633694
Anti-IDE antibody [GT143] used in IHC (Paraffin sections) (IHC-P). GTX633694
Anti-IDE antibody [GT143] used in IHC (Paraffin sections) (IHC-P). GTX633694
Anti-IDE antibody [GT143] used in Western Blot (WB). GTX633694
Anti-IDE antibody [GT143] used in Western Blot (WB). GTX633694

Cat. No. GTX633694

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT143

Isotype

 IgG2b

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IDE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

Positive Control

A431 , mouse brain , rat brain , HeLa

Predict Reactivity

Zebrafish, Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human IDE. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888369

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin degrading enzyme , INSULYSIN

Cellular Localization

Cytoplasm

Background

This gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IDE antibody [GT143] used in Western Blot (WB). GTX633694

GTX633694 WB Image

KO/KD

Wild-type (WT) and IDE knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT143] (GTX633694) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDE antibody [GT143] used in IHC (Paraffin sections) (IHC-P). GTX633694

GTX633694 IHC-P Image

IDE antibody [GT143] detects IDE protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
IDE antibody [GT143] (GTX633694) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IDE antibody [GT143] used in IHC (Paraffin sections) (IHC-P). GTX633694

GTX633694 IHC-P Image

IDE antibody [GT143] detects IDE protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
IDE antibody [GT143] (GTX633694) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IDE antibody [GT143] used in Western Blot (WB). GTX633694

GTX633694 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT143] (GTX633694) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IDE antibody [GT143] used in Western Blot (WB). GTX633694

GTX633694 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT143] (GTX633694) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations