APPLICATION
Application Note
For ELISA: Use at a concentration of 3 - 5μg/ml. Using plates coated with 100 μl/well of the capture antibody (monoclonal anti-human IGFBP3) at 4 μg/ml, in combination with 100 μl/well of a detection antibody (biotinylated IGFBP3) at 400 ng/ml, an ELISA with a range from 125 to 8000 pg/ml can be obtained for sample volumes of 100 ml. For IHC-P: Use at a concentration of 8 - 25 μg/ml on paraffin-embedded tissue sections (5 to 15 μm thick). For WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 41 kDa. Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Product Note
When used with a biotinylated anti human IGFBP3 detection antibody in sandwich ELISAs, this antibody shows less than 0.001 % cross-reactivity with recombinant human IGFI, IGFIR, IGFII, IGFBP1, IGFBP2, IGFBP4, IGFBP5, and IGFBP6.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full length protein (Human).
Conjugation
Unconjugated
RRID
AB_371784
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
insulin like growth factor binding protein 3 , BP-53 , IBP3
Cellular Localization
Secreted
Background
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
REFERENCE
REVIEW
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