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IKK alpha antibody [3G12H9]

Anti-IKK alpha antibody [3G12H9] used in Flow cytometry (FACS). GTX82770
Anti-IKK alpha antibody [3G12H9] used in Western Blot (WB). GTX82770
Anti-IKK alpha antibody [3G12H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82770

Cat. No. GTX82770

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G12H9

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all IKK alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CHUK expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

component of inhibitor of nuclear factor kappa B kinase complex , IKBKA , IKK-alpha , IKK1 , IKKA , NFKBIKA , TCF16

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IKK alpha antibody [3G12H9] used in Flow cytometry (FACS). GTX82770

GTX82770 FACS Image

FACS analysis of A549 cells using GTX82770 IKK alpha antibody [3G12H9].
Blue : IKK alpha
Red : negative control

Anti-IKK alpha antibody [3G12H9] used in Western Blot (WB). GTX82770

GTX82770 WB Image

WB analysis of Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysate using GTX82770 IKK alpha antibody [3G12H9].

Anti-IKK alpha antibody [3G12H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82770

GTX82770 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX82770 IKK alpha antibody [3G12H9].
Green : IKK alpha
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399