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IL13 Receptor alpha 1 antibody

Orthogonal Overexpression
Anti-IL13 Receptor alpha 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105788
Anti-IL13 Receptor alpha 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105788
Anti-IL13 Receptor alpha 1 antibody used in Western Blot (WB). GTX105788
Anti-IL13 Receptor alpha 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105788
Anti-IL13 Receptor alpha 1 antibody used in Western Blot (WB). GTX105788

Cat. No. GTX105788

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IL13 Receptor alpha 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

U87-MG , U87-MG membrane extract , IL13RA1-transfected 293T

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL13 Receptor alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10730754

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 13 receptor subunit alpha 1 , CD213A1 , CT19 , IL-13Ra , NR4

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL13 Receptor alpha 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105788

GTX105788 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using IL13 Receptor alpha 1(GTX105788) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IL13 Receptor alpha 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105788

GTX105788 IHC-P Image

IL13 Receptor alpha 1 antibody detects IL13 Receptor alpha 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
IL13 Receptor alpha 1 stained by IL13 Receptor alpha 1 antibody (GTX105788) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL13 Receptor alpha 1 antibody used in Western Blot (WB). GTX105788

GTX105788 WB Image

Orthogonal

U87-MG whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL13 Receptor alpha 1 antibody (GTX105788) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL13 Receptor alpha 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105788

GTX105788 IHC-P Image

IL13 Receptor alpha 1 antibody detects IL13 Receptor alpha 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
IL13 Receptor alpha 1 stained by IL13 Receptor alpha 1 antibody (GTX105788) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL13 Receptor alpha 1 antibody used in Western Blot (WB). GTX105788

GTX105788 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL13 Receptor alpha 1 antibody (GTX105788) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ding M et al. Calcif Tissue Int 2017; (Epub) A Murine Model for Human ECO Syndrome Reveals a Critical Role of Intestinal Cell Kinase in Skeletal Development.
Ding M et al. bioRxiv 2017; (Epub) Intestinal cell kinase regulates chondrocyte proliferation and maturation during skeletal development.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169