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IP6K1 antibody [GT10611]

Overexpression
Anti-IP6K1 antibody [GT10611] used in Western Blot (WB). GTX628258
Anti-IP6K1 antibody [GT10611] used in IHC (Paraffin sections) (IHC-P). GTX628258
Anti-IP6K1 antibody [GT10611] used in Western Blot (WB). GTX628258

Cat. No. GTX628258

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT10611

Isotype

 IgG2b

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IP6K1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IP6K1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888025

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inositol hexakisphosphate kinase 1 , IHPK1 , PiUS

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a protein that belongs to the inositol phosphokinase (IPK) family. This protein is likely responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). It may also convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4. Alternative splicing occurs for this gene; however, the full-length nature of all transcript variants has not yet been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IP6K1 antibody [GT10611] used in Western Blot (WB). GTX628258

GTX628258 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IP6K1 antibody [GT10611] (GTX628258) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-IP6K1 antibody [GT10611] used in IHC (Paraffin sections) (IHC-P). GTX628258

GTX628258 IHC-P Image

IP6K1 antibody detects IHPK1 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
IP6K1 antibody (GTX628258) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IP6K1 antibody [GT10611] used in Western Blot (WB). GTX628258

GTX628258 WB Image

IP6K1 antibody [GT10611] detects IHPK1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
10 % SDS-PAGE
IP6K1 antibody [GT10611] (GTX628258) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations