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LEF1 antibody, Internal

Anti-LEF1 antibody, Internal used in Western Blot (WB). GTX77879
Anti-LEF1 antibody, Internal used in Western Blot (WB). GTX77879

Cat. No. GTX77879

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
50 μg ($409)
See all LEF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

human fetal heart , Transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human LEF1

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_424827

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lymphoid enhancer binding factor 1 , LEF-1 , TCF10 , TCF1ALPHA , TCF7L3

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-LEF1 antibody, Internal used in Western Blot (WB). GTX77879

GTX77879 WB Image

WB analysis of tranfected 293T cells using GTX77879 LEF1 antibody at 1μg/ml.

Anti-LEF1 antibody, Internal used in Western Blot (WB). GTX77879

GTX77879 WB Image

WB analysis of human fetal heart tissue using GTX77879 LEF1 antibody at 1μg/ml.

REFERENCE

Application Reference

Huang FI et al. Carcinogenesis 2012; 33 (6) : 1142-8 Hepatocyte growth factor activates Wnt pathway by transcriptional activation of LEF1 to facilitate tumor invasion.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409