APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , HeLa nuclear
Predict Reactivity
Mouse, Rat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Lamin A + C. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888110
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1
Cellular Localization
Nucleus
Background
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404](/templates/default/js/lightbox/images/loading.gif "Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404")
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GTX629404 WB Image
Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody [GT9712] (GTX629404) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX629404 ICC/IF Image
Lamin A + C antibody [GT9712] detects Lamin A + C protein at nuclear envelope by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Lamin A + C stained by Lamin A + C antibody [GT9712] (GTX629404) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
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GTX629404 WB Image
Lamin A + C antibody [GT9712] detects Lamin A + C protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
7.5 % SDS-PAGE
Lamin A + C antibody [GT9712] (GTX629404) dilution: 1:1000
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GTX629404 IP Image
Immunoprecipitation of Lamin A + C protein from HeLa whole cell extracts using 5 μg of Lamin A + C antibody [GT9712] (GTX629404).
Western blot analysis was performed using Lamin A + C antibody [GT9712] (GTX629404).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
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REFERENCE
There are currently no references for Lamin A + C antibody [GT9712] (GTX629404). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Lamin A + C antibody [GT9712] (GTX629404). Be the first to share your experience with this product.
![Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404](/upload/website/prouct_img/small/GTX629404/GTX629404_41337_20170323_WB_KO_watermark_w_23061202_347.webp "Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404")
![Anti-Lamin A + C antibody [GT9712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629404](/upload/website/prouct_img/small/GTX629404/GTX629404_44748_20220902_ICC_IF_22101717_764.webp "Anti-Lamin A + C antibody [GT9712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629404")
![Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404](/upload/website/prouct_img/small/GTX629404/GTX629404_41337_WB_Fraction_w_23061202_588.webp "Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404")
![Anti-Lamin A + C antibody [GT9712] used in Immunoprecipitation (IP). GTX629404](/upload/website/prouct_img/small/GTX629404/GTX629404_41337_20151106_IP_w_23061202_926.webp "Anti-Lamin A + C antibody [GT9712] used in Immunoprecipitation (IP). GTX629404")
![Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404](/upload/website/prouct_img/normal/GTX629404/GTX629404_41337_20170323_WB_KO_watermark_w_23061202_347.webp "Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404")
![Anti-Lamin A + C antibody [GT9712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629404](/upload/website/prouct_img/normal/GTX629404/GTX629404_44748_20220902_ICC_IF_22101717_764.webp "Anti-Lamin A + C antibody [GT9712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629404")
![Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404](/upload/website/prouct_img/normal/GTX629404/GTX629404_41337_WB_Fraction_w_23061202_588.webp "Anti-Lamin A + C antibody [GT9712] used in Western Blot (WB). GTX629404")
![Anti-Lamin A + C antibody [GT9712] used in Immunoprecipitation (IP). GTX629404](/upload/website/prouct_img/normal/GTX629404/GTX629404_41337_20151106_IP_w_23061202_926.webp "Anti-Lamin A + C antibody [GT9712] used in Immunoprecipitation (IP). GTX629404")