APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:2000 - 1:10000 |
| 1:1000 - 1:20000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Tris-Citrate/Phosphate
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The immunogen recognized by this antibody maps to a region between residues 300 and 350 of human Minichromosome Maintenance 7 using the numbering given in entry NP_005907.3 (GeneID 4176).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
minichromosome maintenance complex component 7 , CDC47 , MCM2 , P1.1-MCM3 , P1CDC47 , P85MCM , PNAS146 , PPP1R104
Cellular Localization
Nucleus
Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX23732 WB Image
WB analysis of HeLa cell lysate using GTX23732 MCM7 antibody.
Dilution : 0.25 μg/ml
|
|
|
GTX23732 PLA Image
PLA analysis of HeLa cells using GTX23732 MCM7 antibody and anti-human MCM2 antibody.
Red : positive signal
Blue : DAPI
|
|
|
GTX23732 WB Image
WB analysis of HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 cell lysates using GTX23732 MCM7 antibody.
Loading : 50μg
Dilution : 2 μg/ml
|
REFERENCE
There are currently no references for MCM7 antibody (GTX23732). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MCM7 antibody (GTX23732). Be the first to share your experience with this product.
