MED4 antibody [N1], N-term

Cat. No. GTX108541

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all MED4 products

Datasheet
Document

View product citations for antibody GTX108541 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MED4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037399

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mediator complex subunit 4 , ARC36 , DRIP36 , HSPC126 , TRAP36 , VDRIP

Cellular Localization

Nucleus

Background

The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for activation of polymerase II (see MIM 180660)-transcribed genes. MED4 appears to be a core Mediator subunit and is found in nearly all Mediator preparations (summary by Sato et al., 2004 [PubMed 15175163]).[supplied by OMIM]

Database

Gene ID: 29079 MED4
UniProt: Q9NPJ6 MED4

Research Area

DATA IMAGES

GTX108541 WB Image

Sample (30 ug of whole cell lysate)
A: A549 12% SDS PAGE
GTX108541 diluted at 1:1000

GTX108541 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using MED4(GTX108541) antibody at 1:500 dilution.

REFERENCE

There are currently no references for MED4 antibody [N1], N-term (GTX108541). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for MED4 antibody [N1], N-term (GTX108541). Be the first to share your experience with this product.

Submit a Review

SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty