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MEK1/2 (phospho Ser218/222) antibody

Anti-MEK1/2 (phospho Ser218/222) antibody used in Western Blot (WB). GTX48615
Anti-MEK1/2 (phospho Ser218/222) antibody used in Western Blot (WB). GTX48615

Cat. No. GTX48615

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Monkey, Xenopus
Package
100 μl ($499)
See all MEK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM HEPES, 150mM NaCl, 0.01% BSA, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser218,222 of human MEK 1/2. Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_10619879

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 1 , CFC3 , MAPKK1 , MEK1 , MKK1 , PRKMK1

Cellular Localization

Cytoplasm , Nucleus , Membrane

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MEK1/2 (phospho Ser218/222) antibody used in Western Blot (WB). GTX48615

GTX48615 WB Image

Western Blot: MEK1/2 Phosphospecific Antibody [GTX48615] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.

Anti-MEK1/2 (phospho Ser218/222) antibody used in Western Blot (WB). GTX48615

GTX48615 WB Image

REFERENCE

Application Reference

Jau?Tien Lin et al. Journal of Food Biochemistry 2019; (Epub) Litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction exhibited protective efficacy to suppress nickel?induced expression for vascular endothelial growth factor in HepG2 cells.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 499