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MLH1 antibody [G168-728]

Cat. No. GTX01744

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 G168-728

Isotype

 IgG1

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Hamster
Package
500 μl ($419)
See all MLH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:25-1:100
IP Assay dependent

Note :

IHC-P
Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended.
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

Colon carcinoma

PROPERTIES

Form

Liquid

Buffer

Tris-HCl, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant human MLH-1

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mutL homolog 1 , COCA2 , FCC2 , HNPCC , HNPCC2 , hMLH1

Cellular Localization

Nucleus

Background

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]

Database

Research Area

REFERENCE

Clone Reference

2020; Granular dot-like staining with MLH1 immunohistochemistry is a clone-dependent artefact
2019; Screening for microsatellite instability in colorectal carcinoma: Practical utility of immunohistochemistry and PCR with fragment analysis in a diagnostic histopathology setting
Application : IHC-P
Reactivity : Human
2019; Inflammation-Associated Microsatellite Alterations Caused by MSH3 Dysfunction Are Prevalent in Ulcerative Colitis and Increase With Neoplastic Advancement
Application : WB
Reactivity : Human
2019; Downregulation of SPTAN1 is related to MLH1 deficiency and metastasis in colorectal cancer
Application : IHC-P
Reactivity : Human
2007; Physical and functional interactions between Werner syndrome helicase and mismatch-repair initiation factors
Application : IP
Reactivity : Human
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Package List Price ($)
$ 419