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MUC1 antibody [014E]

Anti-MUC1 antibody [014E] used in IHC (Paraffin sections) (IHC-P). GTX57210
Anti-MUC1 antibody [014E] used in Western Blot (WB). GTX57210

Cat. No. GTX57210

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 014E

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all MUC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2,500 - 1:5,000
IHC-P 1:2,500 - 1:5,000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites diluted with PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to common region in Cytoplasmic Tail Domain (CTD) of human MUC1 (CRYVPPSSTDRSPYEKVSAG).

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Mucin 1, Cell Surface Associated , Admckd , Admckd1 , Ca 15-3 , Cd227 , Ema , H23Ag , Kl-6 , Mam6 , Mcd , Mckd , Mckd1 , Muc-1 , Muc-1/Sec , Muc-1/X , Muc1/Zd , Pem , Pemt , Pum , Muc1

Cellular Localization

Apical cell membrane; Single-pass type I membrane protein , Isoform 5: Secreted , Isoform 7: Secreted , Isoform 9: Secreted , Mucin-1 subunit beta: Cell membrane , Cytoplasm , Nucleus.

Background

This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-MUC1 antibody [014E] used in IHC (Paraffin sections) (IHC-P). GTX57210

GTX57210 IHC-P Image

IHC-P analysis of human stomach cancer (Adenocarcinoma Grade I) using GTX57210 MUC1 antibody [014E].
Antigen retrieval : Heat-induced epitope retrieval method (pH6.0 / 30min)
Dilution : 1:2500

Anti-MUC1 antibody [014E] used in Western Blot (WB). GTX57210

GTX57210 WB Image

WB analysis of various samples using GTX57210 MUC1 antibody [014E].
Capan-1 cell lysate: MUC1 positive
HEK293 cell lysate: MUC1 negative
Loading : 5ug per lane
Dilution : 1:2500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 399