APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
| 1:100-1:200 |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 1229 (S-P-Y(p)-E-K) derived from human MUC1.
Purification
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mucin 1, cell surface associated , ADMCKD , ADMCKD1 , CA 15-3 , CD227 , EMA , H23AG , KL-6 , MAM6 , MCD , MCKD , MCKD1 , MUC-1 , MUC-1/SEC , MUC-1/X , MUC1/ZD , PEM , PEMT , PUM
Cellular Localization
Apical cell membrane,Cytoplasm,Nucleus
Background
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
|
GTX55325 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX55325 MUC1 (phospho Tyr1229) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
|
|
|
GTX55325 WB Image
WB analysis of extracts from HepG2 cells treated with PMA using GTX55325 MUC1 (phospho Tyr1229) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
|
|
|
GTX55325 ICC/IF Image
ICC/IF analysis of methanol-fixed HepG2 cells using GTX55325 MUC1 (phospho Tyr1229) antibody.
|
REFERENCE
There are currently no references for MUC1 (phospho Tyr1229) antibody (GTX55325). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MUC1 (phospho Tyr1229) antibody (GTX55325). Be the first to share your experience with this product.
