APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser1943 of human MYH9. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888396
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
myosin heavy chain 9 , BDPLT6 , DFNA17 , EPSTS , FTNS , MATINS , MHA , NMHC-II-A , NMMHC-IIA , NMMHCA
Background
This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955](/templates/default/js/lightbox/images/loading.gif "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955")
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GTX633955 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 (phospho Ser1943) antibody [GT144] (GTX633955) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX633955 WB Image
Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9(phospho Ser1943) antibody [GT144] (GTX633955) diluted at 1:10000.
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GTX633955 IHC-P Image
MYH9 (phospho Ser1943) antibody [GT144] detects MYH9 (phospho Ser1943) protein at cytoplasm in human mammary gland by immunohistochemical analysis.
Sample: Paraffin-embedded human mammary gland.
MYH9 (phospho Ser1943) antibody [GT144] (GTX633955) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633955 IHC-P Image
MYH9 (phospho Ser1943) antibody [GT144] detects MYH9 (phospho Ser1943) protein at cytoplasm in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
MYH9 (phospho Ser1943) antibody [GT144] (GTX633955) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633955 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9(phospho Ser1943) antibody [GT144] (GTX633955) diluted at 1:20000.
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GTX633955 ICC/IF Image
MYH9 (phospho Ser1943) antibody [GT144] detects MYH9 (phospho Ser1943) protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MYH9 (phospho Ser1943) protein stained by MYH9 (phospho Ser1943) antibody [GT144] (GTX633955) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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REFERENCE
There are currently no references for MYH9 (phospho Ser1943) antibody [GT144] (GTX633955). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MYH9 (phospho Ser1943) antibody [GT144] (GTX633955). Be the first to share your experience with this product.
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955](/upload/website/prouct_img/small/GTX633955/GTX633955_42723_20180817_WB_shRNA_watermark_w_23061202_719.webp "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955")
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955](/upload/website/prouct_img/small/GTX633955/GTX633955_42723_20170209_WB_CIP_w_23061202_883.webp "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955")
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in IHC (Paraffin sections) (IHC-P). GTX633955](/upload/website/prouct_img/small/GTX633955/GTX633955_42723_20170405_IHC-P_2_w_23061202_897.webp "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in IHC (Paraffin sections) (IHC-P). GTX633955")
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in IHC (Paraffin sections) (IHC-P). GTX633955](/upload/website/prouct_img/small/GTX633955/GTX633955_42723_20170405_IHC-P_1_w_23061202_379.webp "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in IHC (Paraffin sections) (IHC-P). GTX633955")
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955](/upload/website/prouct_img/small/GTX633955/GTX633955_42723_20170209_WB_w_23061202_790.webp "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955")
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633955](/upload/website/prouct_img/small/GTX633955/GTX633955_42723_20170419_IFA_w_23061202_522.webp "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633955")
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955](/upload/website/prouct_img/normal/GTX633955/GTX633955_42723_20180817_WB_shRNA_watermark_w_23061202_719.webp "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955")
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955](/upload/website/prouct_img/normal/GTX633955/GTX633955_42723_20170209_WB_CIP_w_23061202_883.webp "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955")
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in IHC (Paraffin sections) (IHC-P). GTX633955](/upload/website/prouct_img/normal/GTX633955/GTX633955_42723_20170405_IHC-P_2_w_23061202_897.webp "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in IHC (Paraffin sections) (IHC-P). GTX633955")
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in IHC (Paraffin sections) (IHC-P). GTX633955](/upload/website/prouct_img/normal/GTX633955/GTX633955_42723_20170405_IHC-P_1_w_23061202_379.webp "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in IHC (Paraffin sections) (IHC-P). GTX633955")
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955](/upload/website/prouct_img/normal/GTX633955/GTX633955_42723_20170209_WB_w_23061202_790.webp "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Western Blot (WB). GTX633955")
![Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633955](/upload/website/prouct_img/normal/GTX633955/GTX633955_42723_20170419_IFA_w_23061202_522.webp "Anti-MYH9 (phospho Ser1943) antibody [GT144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633955")