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Merlin (phospho Ser518) antibody

Orthogonal
Anti-Merlin (phospho Ser518) antibody used in Western Blot (WB). GTX22478
Anti-Merlin (phospho Ser518) antibody used in Western Blot (WB). GTX22478

Cat. No. GTX22478

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, Multiplexing

Reactivity

 Mouse
Package
50 μg ($399)
See all Merlin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA 1:200000
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Product Note

This phospho-specific polyclonal antibody is specific for phosphorylated pS518 of human NF2 (neurofibromatosis 2 gene product).   Reactivity with non-phosphorylated Human NF2 (Merlin) is minimal by ELISA.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to KLH.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofibromin 2 , ACN , BANF , SCH

Cellular Localization

Nucleus,Cytoplasm, cytoskeleton

Background

The recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (Merlin). The protein product Merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence results in the occurrence of multiple tumors of the nervous system, particularly schwannomas and meningiomas. Merlin's similarity to ERMs suggests that it might share functions, acting as a link between cytoskeletal components and the cell membrane. The NF2 protein is highly expressed in human fibroblasts and detected as a single band of about 75kD. This antibody is specific for the phosphorylated form of human NF2 (Merlin).

Database

Research Area

DATA IMAGES

Anti-Merlin (phospho Ser518) antibody used in Western Blot (WB). GTX22478

GTX22478 WB Image

Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 (GTX22478) was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amersham's substrate system. Other detection methods will yield similar results.

Anti-Merlin (phospho Ser518) antibody used in Western Blot (WB). GTX22478

GTX22478 WB Image

Orthogonal

WB analysis of mouse cardiac myocyte lysate using GTX22478 Merlin (phospho Ser518) antibody.
Lane 1 : Mock
Lane 2 : 4 nM CLA-treated cells
Lane 3 : 20 nM CLA-treated cells
Lane 4 : 100 nM CLA-treated cells
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399