APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-3μg/ml |
1-2μg/ml |
Note :
IHC-P
Human Thymus shows cytoplasm staining in select areas.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89269-PEP).
Predict Reactivity
Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-ETQKHKKDLHPLFD, from the internal region of the protein sequence according to NP_954712.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
unc-13 homolog D , FHL3 , HLH3 , HPLH3 , Munc13-4
Cellular Localization
Cytoplasm , Lysosome
Background
This gene encodes a protein that is a member of the UNC13 family, containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis type 3, a genetically heterogeneous, rare autosomal recessive disorder. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89269 IHC-P Image
IHC-P analysis of human thymus using GTX89269 Munc 13-4 antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 2.5μg/ml
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GTX89269 WB Image
WB analysis of human T-lymphocyte lysate using GTX89269 Munc 13-4 antibody, Internal. Dilution : 2μg/ml Loading : 35μg protein in RIPA buffer
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GTX89269 IHC-P Image
IHC-P analysis of mouse lung (wt in A and KO in B) using GTX89269 Munc 13-4 antibody, Internal. Dilution : 0.5μg/ml
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REFERENCE
There are currently no references for Munc 13-4 antibody, Internal (GTX89269). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Munc 13-4 antibody, Internal (GTX89269). Be the first to share your experience with this product.