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OTUD6B antibody

Anti-OTUD6B antibody used in Western Blot (WB). GTX122242
Anti-OTUD6B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122242

Cat. No. GTX122242

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all OTUD6B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human OTUD6B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175755

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

OTU domain containing 6B , CGI-77 , DUBA-5 , DUBA5 , IDDFSDA

Background

Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA5 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-OTUD6B antibody used in Western Blot (WB). GTX122242

GTX122242 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX122242 diluted at 1:1000

Anti-OTUD6B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122242

GTX122242 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using OTUD6B(GTX122242) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169