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PAX6 antibody [HL2599]

Recombinant antibody Orthogonal Overexpression
Anti-PAX6 antibody [HL2599] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639051
Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051
Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051
Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051
Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051
Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051

Cat. No. GTX639051

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2599

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all PAX6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Product Note

This antibody is specific for human PAX6 protein, and it does not cross react with human PAX2, PAX4, PAX5, and PAX8 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of Human PAX6.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paired box 6 , AN , AN2 , ASGD5 , D11S812E , FVH1 , MGDA , WAGR

Cellular Localization

Nucleus

Background

This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]

Database

Research Area

DATA IMAGES

Anti-PAX6 antibody [HL2599] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639051

GTX639051 ICC/IF Image

PAX6 antibody [HL2599] detects PAX6 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PAX6 stained by PAX6 antibody [HL2599] (GTX639051) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051

GTX639051 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051

GTX639051 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051

GTX639051 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051

GTX639051 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PAX6 antibody [HL2599] used in Western Blot (WB). GTX639051

GTX639051 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations