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PDI antibody [1D3]

Anti-PDI antibody [1D3] used in Immunohistochemistry (IHC). GTX30716
Anti-PDI antibody [1D3] used in Western Blot (WB). GTX30716

Cat. No. GTX30716

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1D3

Isotype

 IgG1

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse, Rat, Sheep, Bovine, Dog, Hamster, Guinea pig, Pig, Monkey, Xenopus laevis
Package
50 μg ($399)
See all PDI products

APPLICATION

Application Note

For ICC: Use at a concentration of 2-5 μg/ml. For IP: Use at a concentration of 8-12 μg/ml. For WB: Use at a concentration of 2-4 μg/ml. Predicted molecular weight: 58 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol, 0.1mM PMSF

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide corresponding to amino acids 499-509 of rat protein disulfide isomerase conjugated to KLH.

Purification

Protein G affinity purified

Conjugation

Unconjugated

RRID

AB_1241484

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Prolyl 4-Hydroxylase Subunit Beta , Pdi , Pdir , P4Hb

Background

The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Research Area

DATA IMAGES

Anti-PDI antibody [1D3] used in Immunohistochemistry (IHC). GTX30716

GTX30716 IHC Image

Immunohistochemistry analysis of human HEK293 cells transfected with wild type TorsinA co-stained with PDI mAb (1D3) (red). Co-localization of TorsinA and PDI proteins merge as yellow.

Anti-PDI antibody [1D3] used in Western Blot (WB). GTX30716

GTX30716 WB Image

Western blot analysis of PDI:
Lane 1: MW marker
Lane 2: HeLa (HS)
Lane 3: ADI-SPP-891
Lane 4: Vero (HS)
Lane 5: 3T3 (HS)
Lane 6: PC-12 (HS)
Lane 7: CHO-K1 (HS)

REFERENCE

Application Reference

Hansen FM et al. Life Sci Alliance 2024; 7 (2) Mitochondrial phosphoproteomes are functionally specialized across tissues.
Sassano ML et al. J Cell Biol 2023; 222 (3) PERK recruits E-Syt1 at ER-mitochondria contacts for mitochondrial lipid transport and respiration.
Reactivity : Human
Guyard V et al. J Cell Biol 2022; 221 (9) ORP5 and ORP8 orchestrate lipid droplet biogenesis and maintenance at ER-mitochondria contact sites.
Leila Rochin et al. bioRxiv 2019; (Epub) ORP5 Regulates Transport of Lipids and Calcium to Mitochondria at Endoplasmic Reticulum-Mitochondria Membrane Contact Sites.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399