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PKA C alpha antibody

Anti-PKA C alpha antibody used in IHC (Whole mount) (IHC-Wm). GTX107567
Anti-PKA C alpha antibody used in Western Blot (WB). GTX107567
Anti-PKA C alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107567
Anti-PKA C alpha antibody used in Western Blot (WB). GTX107567

Cat. No. GTX107567

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all PKA C alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

NIH-3T3 , C2C12 , HeLaS3

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKA C alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951392

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase cAMP-activated catalytic subunit alpha , PKACA , PPNAD4

Cellular Localization

Cytoplasm , Nucleus

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PKA C alpha antibody used in IHC (Whole mount) (IHC-Wm). GTX107567

GTX107567 IHC-Wm Image

PKA C alpha antibody detects PKA C alpha protein at spinal cord neurons and muscles on whole-mount zebrafish embryos by immunohistochemical analysis.?
Sample: Paraformaldehyde-fixed zebrafish embryos.
PKA C alpha antibody (GTX107567) dilution: 1:200.

Anti-PKA C alpha antibody used in Western Blot (WB). GTX107567

GTX107567 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
12% SDS PAGE
GTX107567 diluted at 1:500

Anti-PKA C alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107567

GTX107567 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PKA alpha(GTX107567) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-PKA C alpha antibody used in Western Blot (WB). GTX107567

GTX107567 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: C2C12
10% SDS PAGE
GTX107567 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169