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PKC alpha (phospho Thr497) antibody

Anti-PKC alpha (phospho Thr497) antibody used in Western Blot (WB). GTX52316

Cat. No. GTX52316

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all PKC alpha products

APPLICATION

Application Note

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PKC-pan around the phosphorylation site of Thr497

Purification

Antigen affinity purification

Conjugation

Unconjugated

RRID

AB_2887790

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES

Anti-PKC alpha (phospho Thr497) antibody used in Western Blot (WB). GTX52316

GTX52316 WB Image

Western blot analysis of PKC alpha phosphorylation expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

REFERENCE

Application Reference

Iannotti FA et al. Nat Commun 2018; 9 (1) : 3950 Genetic and pharmacological regulation of the endocannabinoid CB1 receptor in Duchenne muscular dystrophy.
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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399