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PKC beta 1 (phospho Ser661) antibody

Anti-PKC beta 1 (phospho Ser661) antibody used in Western Blot (WB). GTX04593
Anti-PKC beta 1 (phospho Ser661) antibody used in IHC (Paraffin sections) (IHC-P). GTX04593
Anti-PKC beta 1 (phospho Ser661) antibody used in Western Blot (WB). GTX04593
Anti-PKC beta 1 (phospho Ser661) antibody used in Western Blot (WB). GTX04593
Anti-PKC beta 1 (phospho Ser661) antibody used in ELISA (ELISA). GTX04593

Cat. No. GTX04593

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Predict Reactivity

Zebrafish, Dog, Chicken, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PRKCB(Accession P05771), corresponding to amino acid residues around phosphorylated Ser661.

Purification

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C beta , PKC-beta , PKCB , PKCI(2) , PKCbeta , PRKCB1 , PRKCB2

Cellular Localization

Cytoplasm , Nucleus , Membrane

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKC beta 1 (phospho Ser661) antibody used in Western Blot (WB). GTX04593

GTX04593 WB Image

WB analysis of mouse brain tissue lysate using GTX04593 PKC beta 1 (phospho Ser661) antibody. The lane on the left was treated with blocking peptide.

Anti-PKC beta 1 (phospho Ser661) antibody used in IHC (Paraffin sections) (IHC-P). GTX04593

GTX04593 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX04593 PKC beta 1 (phospho Ser661) antibody.
Antigen retrieval : Heat mediated citrate buffer.
Dilution : 1:200

Anti-PKC beta 1 (phospho Ser661) antibody used in Western Blot (WB). GTX04593

GTX04593 WB Image

WB analysis of P19 whole cell lysate with heat-shock treatment using GTX04593 PKC beta 1 (phospho Ser661) antibody. The lane on the left was treated with blocking peptide.

Anti-PKC beta 1 (phospho Ser661) antibody used in Western Blot (WB). GTX04593

GTX04593 WB Image

WB analysis of Raw264.7 whole cell lysate with LPS-treatment using GTX04593 PKC beta 1 (phospho Ser661) antibody. The lane on the left was treated with blocking peptide.

Anti-PKC beta 1 (phospho Ser661) antibody used in ELISA (ELISA). GTX04593

GTX04593 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX04593 PKC beta 1 (phospho Ser661) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1 μg/mL

REFERENCE

There are currently no references for PKC beta 1 (phospho Ser661) antibody (GTX04593). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PKC beta 1 (phospho Ser661) antibody (GTX04593). Be the first to share your experience with this product.
Package List Price ($)
$ 399