APPLICATION
Application Note
Calculated MW
Positive Control
NIH-3T3 , NIH-3T3 (20 ng/ml TNF-alpha treatment for 30 min) , NIH-3T3 (starvation for 16 hr, 100 ng/ml PMA treatment for 20 min) , NIH-3T3 (starvation for 16 hr, 5 ng/ml PDGF treatment for 15 min)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic phosphopeptide derived from human PKC θ around the phosphorylation site of Threonine 538.
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Protein Kinase C Theta , Prkct , Npkc-Theta , Prkcq
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]
Research Area
DATA IMAGES
|
GTX20304 WB Image
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Thr538) antibody (GTX20304) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX20304 WB Image
WB analysis of various samples using GTX20304 PKC theta (phospho Thr538) antibody.
Lane1 : The Spleen tissue lysate of Rat
Lane2 : The Spleen tissue lysate of Mouse
Lane3 : L02 treated with LPS(100ng/ml) for 30 minutes whole cell lysate
Lane4 : HEK293T treated with Etoposide(20ng/ml) for 6 hours whole cell lysate
Lane5 : HEK293T treated with TNFα(20ng/ml)for 20 minutes whole cell lysate
Dilution : 1:500
Loading : 40ug
|
|
|
GTX20304 WB Image
WB analysis of various samples using GTX20304 PKC theta (phospho Thr538) antibody.
Lane1 : MCF-7 whole cell lysate
Lane2 : MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate
Lane3 : MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate
Dilution : 1:500
|
REFERENCE
There are currently no references for PKC theta (phospho Thr538) antibody (GTX20304). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PKC theta (phospho Thr538) antibody (GTX20304). Be the first to share your experience with this product.
