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PLK4 antibody, N-term

Anti-PLK4 antibody, N-term used in Western Blot (WB). GTX22642
Anti-PLK4 antibody, N-term used in Flow cytometry (FACS). GTX22642
Anti-PLK4 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22642
Anti-PLK4 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22642

Cat. No. GTX22642

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μg ($399)
See all PLK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
ICC/IF 10μg/ml
FACS Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells.
Not tested in other applications.

Calculated MW

109 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX22642-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence ATCIGEKIEDFKVGN, from the N Terminus of the protein sequence according to NP_055079.3, NP_001177728.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polo like kinase 4 , MCCRP2 , SAK , STK18

Cellular Localization

Nucleolus

Background

This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-PLK4 antibody, N-term used in Western Blot (WB). GTX22642

GTX22642 WB Image

WB analysis of human colon lysate using GTX22642 PLK4 antibody, N-term.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer

Anti-PLK4 antibody, N-term used in Flow cytometry (FACS). GTX22642

GTX22642 FACS Image

FACS analysis of PFA fixed HeLa cells using GTX22642 PLK4 antibody, N-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

Anti-PLK4 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22642

GTX22642 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX22642 PLK4 antibody, N-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-PLK4 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22642

GTX22642 ICC/IF Image

ICC/IF analysis of PFA fixed A431 cells using GTX22642 PLK4 antibody, N-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399