APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.2-2.5 ug/ml |
2-10 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
Hamster CHO K1 , HEK273 , HeLa , HepG2 , Human skin fibroblasts
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a N-terminal region of Human PPAT
Purification
Affinity Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphoribosyl pyrophosphate amidotransferase , ATASE , GPAT , PRAT
Background
The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
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GTX46492 WB Image
WB analysis of HepG2 cells using GTX46492 PPAT antibody at 0.25μg/ml.
Lane A : marker
Lane B : HepG2 cells
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GTX46492 WB Image
WB analysis of human skin fibroblasts, HepG2, HEK273 cells, HeLa cells, and hamster CHO K1 cells using GTX46492 PPAT antibody at 1:5000.
Lane :
1 :Human skin fibroblast (100μg)
2 : HepG2 (20μg)
3 : HEK293 (20μg)
4 : HeLa (20μg)
5 : Hamster CHO K1 (20μg)
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GTX46492 IHC-P Image
IHC-P analysis of human kidney tissue using GTX46492 PPAT antibody at 4.0-8.0μg/ml.
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REFERENCE
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REVIEW
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