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PRX III antibody [C2C3], C-term

KO/KD
Anti-PRX III antibody [C2C3], C-term used in Western Blot (WB). GTX111887
Anti-PRX III antibody [C2C3], C-term used in Western Blot (WB). GTX111887
Anti-PRX III antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111887
Anti-PRX III antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111887

Cat. No. GTX111887

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PRX III products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , HeLa

Predict Reactivity

Mouse, Rat, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PRX III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037652

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxiredoxin 3 , AOP-1 , AOP1 , HBC189 , MER5 , PRO1748 , SP-22 , prx-III

Cellular Localization

Mitochondrion

Background

This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRX III antibody [C2C3], C-term used in Western Blot (WB). GTX111887

GTX111887 WB Image

KO/KD

Wild-type (WT) and PRX III knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX III antibody [C2C3], C-term (GTX111887) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PRX III antibody [C2C3], C-term used in Western Blot (WB). GTX111887

GTX111887 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX111887 diluted at 1:1000

Anti-PRX III antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111887

GTX111887 ICC/IF Image

Peroxiredoxin 3 antibody [C2C3], C-term detects Peroxiredoxin 3 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: Peroxiredoxin 3 protein stained by Peroxiredoxin 3 antibody [C2C3], C-term (GTX111887) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PRX III antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111887

GTX111887 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using PRX III(GTX111887) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169