Application Note
Western blot. Recommended dilution for Western blot 1:500-1:1,000. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Product Note
Reacts with residues 167-189 (AIVEALNGKEVAAQVKAPLVLKD) of human PARK7 (DJ-1). Also detects PARK7 (DJ-1) in mouse brain. 82% sequence identity to mouse and rat DJ-1.
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Peptide: AIVEALNGKEVAAQVKAPLVLKD
Purification
Affinity purified with antigen.
From polyclonal serum.
Conjugation
Unconjugated
RRID
AB_424599
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Background
Four chromosomal loci (PARK2, PARK6, PARK7, and PARK9) associated with autosomal recessive, early onset parkinsonism are known. Positional cloning within the refined PARK7 critical region recently identified mutations in the DJ-1 gene in PARK7-linked families. Mutations in the PARK7/DJ-1 gene cause autosomal-recessive Parkinsons disease.
Research Area