APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1/500 - 1/2000 |
1/200 - 1/1000 |
1/200 - 1/400 |
1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human RSK2 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ribosomal protein S6 kinase A3 , CLS , HU-3 , ISPK-1 , MAPKAPK1B , MRX19 , RSK , RSK2 , S6K-alpha3 , p90-RSK2 , pp90RSK2
Cellular Localization
Nucleus,Cytoplasm
Background
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX60517 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX60517 RSK2 antibody [4E10]. Green : RSK2 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
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GTX60517 WB Image
WB analysis of human RSK2 (AA: 1-212) recombinant protein using GTX60517 RSK2 antibody [4E10].
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GTX60517 ELISA Image
ELISA analysis of antigen using GTX60517 RSK2 antibody [4E10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60517 FACS Image
FACS analysis of HepG2 cells using GTX60517 RSK2 antibody [4E10]. Green : RSK2 Red : negative control
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GTX60517 WB Image
WB analysis of HeLa (1), MCF-7 (2), and HepG2 (3) cell lysate using GTX60517 RSK2 antibody [4E10].
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REFERENCE
There are currently no references for RSK2 antibody [4E10] (GTX60517). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RSK2 antibody [4E10] (GTX60517). Be the first to share your experience with this product.