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Rho GDI alpha antibody [1A7]

KO/KD Overexpression
Anti-Rho GDI alpha antibody [1A7] used in IHC (Paraffin sections) (IHC-P). GTX84860
Anti-Rho GDI alpha antibody [1A7] used in IHC (Paraffin sections) (IHC-P). GTX84860
Anti-Rho GDI alpha antibody [1A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84860
Anti-Rho GDI alpha antibody [1A7] used in Western Blot (WB). GTX84860
Anti-Rho GDI alpha antibody [1A7] used in IHC (Paraffin sections) (IHC-P). GTX84860
Anti-Rho GDI alpha antibody [1A7] used in Western Blot (WB). GTX84860

Cat. No. GTX84860

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1A7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all Rho GDI alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ARHGDIA(NP_004300) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho GDP dissociation inhibitor alpha , GDIA1 , HEL-S-47e , NPHS8 , RHOGDI , RHOGDI-1

Cellular Localization

Cytoplasm

Background

Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.

Database

  • Gene ID: 396 ARHGDIA
  • UniProt: P52565 ARHGDIA

Research Area

DATA IMAGES

Anti-Rho GDI alpha antibody [1A7] used in IHC (Paraffin sections) (IHC-P). GTX84860

GTX84860 IHC-P Image

IHC-P analysis of kidney tissue using GTX84860 Rho GDI alpha antibody [1A7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Rho GDI alpha antibody [1A7] used in IHC (Paraffin sections) (IHC-P). GTX84860

GTX84860 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84860 Rho GDI alpha antibody [1A7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Rho GDI alpha antibody [1A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84860

GTX84860 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Rho GDI alpha plasmid using GTX84860 Rho GDI alpha antibody [1A7].

Anti-Rho GDI alpha antibody [1A7] used in Western Blot (WB). GTX84860

GTX84860 WB Image

KO/KD

WB analysis of wild type and Rho GDI alpha knock out 293T cell lysate(10μg per lane) using GTX84860 Rho GDI alpha antibody [1A7].
Dilution : 1:200

Anti-Rho GDI alpha antibody [1A7] used in IHC (Paraffin sections) (IHC-P). GTX84860

GTX84860 IHC-P Image

IHC-P analysis of prostate tissue using GTX84860 Rho GDI alpha antibody [1A7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Rho GDI alpha antibody [1A7] used in Western Blot (WB). GTX84860

GTX84860 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Rho GDI alpha plasmid (Right) or empty vector (Left) for 48 hrs using GTX84860 Rho GDI alpha antibody [1A7].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519