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RhoA antibody

Anti-RhoA antibody used in Western Blot (WB). GTX66647
Anti-RhoA antibody used in IHC (Paraffin sections) (IHC-P). GTX66647

Cat. No. GTX66647

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RhoA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 150-200 of Human Rho A.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ras homolog family member A , ARH12 , ARHA , RHO12 , RHOH12

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-RhoA antibody used in Western Blot (WB). GTX66647

GTX66647 WB Image

WB analysis of various samples using GTX66647 RhoA antibody.
Lane1 : HEK293T whole cell lysate
Lane2 : Raw264.7 whole cell lysate
Lane3 : H9C2 whole cell lysate
Dilution : 1:500

Anti-RhoA antibody used in IHC (Paraffin sections) (IHC-P). GTX66647

GTX66647 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX66647 RhoA antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399